Resveratrol protects against copper and iron toxicity in Drosophila melanogaster

BackgroundCopper (Cu) and iron (Fe) are essential trace elements that when in excess capable of causing cytotoxic effects leading to lipid peroxidation promoting oxidative stress. Resveratrol (RES) is a natural polyphenol with antioxidant anti-inflammatory properties. This study was carried out evaluate the protective role RES Fe Cu sulphate-induced stress Drosophila melanogaster. MethodsAdult wild type flies were fed Cu2+ Fe2+ (1 mM each) and/or (30 60 mg/kg diet) for 7 days. Survival, negative geotaxis emergence rate evaluated by daily recording fruit fly mortality final analysis. Fruit anaesthetized using CO2 gas, homogenized centrifuged at 4,000 rpm 10 minutes 4 °C. Aliquots supernatants used estimation biochemical markers spectrophotometry. ResultsFruit co-treated FeSO4 + CuSO4 mg/Kg) significantly elevated H2O2, NO, peroxidation, acetylcholinesterase as well GSH, GST, catalase total thiols (p<0.05) compared (1mM treated group. Flies 60mg/Kg) also had improved (p< 0.05) eclosion climbing rates ConclusionThis demonstrated reduced Fe2+-induced radical generation D. melanogaster buffering capability flies. Therefore, could be management disorders involving